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Mitochondrial Fission Factor (MFF) Antibody

ABX030876
Abbexa
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
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Overview

  • Supplier
    Abbexa
  • Product Name
    Mitochondrial Fission Factor (MFF) Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate All lanes: MFF antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 33 28, 26 kDa Observed band size: 35 kDa
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Figure 1. Western blot analysis of MFF using anti-MFF antibody (A02563-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human MOLT-4 whole clel lysates, Lane 5: human HEL whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFF antigen affinity purified polyclonal antibody (Catalog # A02563-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MFF at approximately 26 kDa. The expected band size for MFF is at 26 kDa.
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