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Anti-MGP Antibody

144-65913
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMGP
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-MGP Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID4256
  • Target name
    MGP
  • Target description
    matrix Gla protein
  • Target synonyms
    GIG36, MGLAP, NTI, matrix Gla protein, cell growth-inhibiting gene 36 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08493
  • Protein Name
    Matrix Gla protein
  • Scientific Description
    MGP pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MGP using anti-MGP antibody (PB9954). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: JURKAT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGP antigen affinity purified polyclonal antibody (Catalog # PB9954) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGP at approximately 12 kDa. The expected band size for MGP is at 12 kDa.
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