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Immunohistochemical staining of human soft tissue shows strong cytoplasmic positivity in chondrocytes.
Immunohistochemical staining of human soft tissue shows strong cytoplasmic positivity in chondrocytes.
Immunohistochemical staining of human soft tissue shows strong cytoplasmic positivity in chondrocytes.

Anti-MGP Antibody

HPA013949
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMGP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MGP
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4256
  • Target name
    MGP
  • Target description
    matrix Gla protein
  • Target synonyms
    cell growth-inhibiting gene 36 protein; GIG36; matrix Gla protein; MGLAP; NTI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08493
  • Protein Name
    Matrix Gla protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA013789LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of MGP using anti-MGP antibody (PB9954). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: JURKAT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGP antigen affinity purified polyclonal antibody (Catalog # PB9954) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGP at approximately 12 kDa. The expected band size for MGP is at 12 kDa.
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