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Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.

Anti-MIB1 Antibody

HPA019100
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetMIB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MIB1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57534
  • Target name
    MIB1
  • Target description
    MIB E3 ubiquitin protein ligase 1
  • Target synonyms
    DAPK-interacting protein 1; DIP1; DIP-1; E3 ubiquitin-protein ligase MIB1; LVNC7; MIB; mindbomb E3 ubiquitin protein ligase 1; RING-type E3 ubiquitin transferase MIB1; ubiquitin ligase mind bomb; zinc finger ZZ type with ankyrin repeat domain protein 2; ZZANK2; ZZZ6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86YT6
  • Protein Name
    E3 ubiquitin-protein ligase MIB1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Mib1 using anti-Mib1 antibody (A01387-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mib1 antigen affinity purified polyclonal antibody (Catalog # A01387-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mib1 at approximately 110 kDa. The expected band size for Mib1 is at 110 kDa.
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