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Western blot All lanes: MIB1 antibody at 2microg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 111 kDa Observed band size: 111 kDa
Western blot All lanes: MIB1 antibody at 2microg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 111 kDa Observed band size: 111 kDa
Western blot All lanes: MIB1 antibody at 2microg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 111 kDa Observed band size: 111 kDa

MIB1 Antibody

CSB-PA13289A0RB
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMIB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MIB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57534
  • Target name
    MIB1
  • Target description
    MIB E3 ubiquitin protein ligase 1
  • Target synonyms
    DAPK-interacting protein 1; DIP1; DIP-1; E3 ubiquitin-protein ligase MIB1; LVNC7; MIB; mindbomb E3 ubiquitin protein ligase 1; RING-type E3 ubiquitin transferase MIB1; ubiquitin ligase mind bomb; zinc finger ZZ type with ankyrin repeat domain protein 2; ZZANK2; ZZZ6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86YT6
  • Protein Name
    E3 ubiquitin-protein ligase MIB1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Mib1 using anti-Mib1 antibody (A01387-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mib1 antigen affinity purified polyclonal antibody (Catalog # A01387-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mib1 at approximately 110 kDa. The expected band size for Mib1 is at 110 kDa.
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