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Anti-MICB

A3092
Selleck Chemicals
Product group Antibodies
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Overview

  • Supplier
    Selleck Chemicals
  • Product Name
    Anti-MICB
  • Delivery Days Customer
    9
  • Certification
    Research Use Only
  • Estimated Purity
    ...
  • Scientific Description
    Anti-MICB is an antibody that specifically target human major histocompatibility complex class I chain-related A and B (MICA/B). It can be used in treating a cancer. MW :143.66 KD.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MICB using anti-MICB antibody (PB9613). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human SiHa whole cell lysates, Lane 7: human HEPA1/6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MICB antigen affinity purified polyclonal antibody (Catalog # PB9613) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MICB at approximately 60-65 kDa. The expected band size for MICB is at 43 kDa.
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