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Anti-MIEF1 Antibody

Anti-MIEF1 Antibody

A8604
Antibodies.com
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman

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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-MIEF1 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to MIEF1.
  • Reactivity
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MIEF1 using anti-MIEF1 antibody (A06887-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human U-937 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: rat heart tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIEF1 antigen affinity purified polyclonal antibody (Catalog # A06887-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIEF1 at approximately 51KD. The expected band size for MIEF1 is at 51KD.
Product group Antibodies
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Anti-MIEF1 Antibody Picoband(r)A06887-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMIEF1
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Mouse kidney lysates probed with SMCR7L Polyclonal Antibody, Unconjugated (bs-12634R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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MIEF1 Polyclonal AntibodyBS-12634R
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