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IHC-P analysis of mouse kidney tissue using GTX03170 SMCR7L antibody. Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed Dilution : 1:100
IHC-P analysis of mouse kidney tissue using GTX03170 SMCR7L antibody. Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed Dilution : 1:100
IHC-P analysis of mouse kidney tissue using GTX03170 SMCR7L antibody. Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed Dilution : 1:100

SMCR7L antibody

GTX03170
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMIEF1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SMCR7L antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID54471
  • Target name
    MIEF1
  • Target description
    mitochondrial elongation factor 1
  • Target synonyms
    AltMIEF1, D3A, HSU79252, L0R8F8, MID51, MIEF1-MP, OPA14, SMCR7L, dJ1104E15.3, mitochondrial dynamics protein MIEF1, DANGER family member 3A, MIEF1 microprotein, SMCR7-like protein, Smith-Magenis syndrome chromosome region, candidate 7-like, alternative MIEF1 protein, alternative protein SMCR7L, mitochondrial dynamic protein MID51, mitochondrial dynamic protein of 51 kDa, mitochondrial dynamics protein MID51, mitochondrial dynamics protein of 51 kDa, smith-Magenis syndrome chromosomal region candidate gene 7 protein-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NQG6
  • Protein Name
    Mitochondrial dynamics protein MIEF1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MIEF1 using anti-MIEF1 antibody (A06887-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human U-937 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: rat heart tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIEF1 antigen affinity purified polyclonal antibody (Catalog # A06887-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIEF1 at approximately 51KD. The expected band size for MIEF1 is at 51KD.
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