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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-MINDY3 Antibody

HPA038400
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMINDY3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MINDY3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80013
  • Target name
    MINDY3
  • Target description
    MINDY lysine 48 deubiquitinase 3
  • Target synonyms
    C10orf97, CARP, DERP5, FAM188A, MST126, MSTP126, my042, ubiquitin carboxyl-terminal hydrolase MINDY-3, CARD-containing protein, MINDY deubiquitinase 3, caspase recruitment domain containing pro-apoptotic protein, dermal papilla-derived protein 5, deubiquitinating enzyme MINDY-3, family with sequence similarity 188 member A, protein FAM188A, ubiquitin carboxyl-terminal hydrolase FAM188A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H8M7
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase MINDY-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MINDY3 using anti-MINDY3 antibody (A32140-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human SH-SY5Y whole cell lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MINDY3 antigen affinity purified polyclonal antibody (Catalog # A32140-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MINDY3 at approximately 50 kDa. The expected band size for MINDY3 is at 50 kDa.
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