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ProductsBack/FAM188A / C10orf97 Antibody (aa51-100)

FAM188A / C10orf97 Antibody (aa51-100)

LS-C170388
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMINDY3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FAM188A / C10orf97 Antibody (aa51-100)
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID80013
  • Target name
    MINDY3
  • Target description
    MINDY lysine 48 deubiquitinase 3
  • Target synonyms
    C10orf97; CARD-containing protein; CARP; caspase recruitment domain containing pro-apoptotic protein; dermal papilla-derived protein 5; DERP5; deubiquitinating enzyme MINDY-3; FAM188A; family with sequence similarity 188 member A; MINDY deubiquitinase 3; MST126; MSTP126; my042; protein FAM188A; ubiquitin carboxyl-terminal hydrolase FAM188A; ubiquitin carboxyl-terminal hydrolase MINDY-3
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MINDY3 using anti-MINDY3 antibody (A32140-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human SH-SY5Y whole cell lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MINDY3 antigen affinity purified polyclonal antibody (Catalog # A32140-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MINDY3 at approximately 50 kDa. The expected band size for MINDY3 is at 50 kDa.
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