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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Anti-MINDY4 Antibody

HPA019475
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetMINDY4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MINDY4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84182
  • Target name
    MINDY4
  • Target description
    MINDY lysine 48 deubiquitinase 4
  • Target synonyms
    AQP1; AQP-1; Aquaporin-1; Aquaporin-CHIP; C7orf67; CHIP28; FAM188B; family with sequence similarity 188 member B; MINDY deubiquitinase 4; probable deubiquitinating enzyme MINDY-4; probable ubiquitin carboxyl-terminal hydrolase FAM188B; probable ubiquitin carboxyl-terminal hydrolase MINDY-4; protein FAM188B; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ4G0A6
  • Protein Name
    Probable ubiquitin carboxyl-terminal hydrolase MINDY-4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human kidney shows strong membranous and cytoplasmic positivity in subsets of tubules.
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Figure 1. Western blot analysis of MINDY4 using anti-MINDY4 antibody (A32449-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MINDY4 antigen affinity purified polyclonal antibody (Catalog # A32449-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MINDY4 at approximately 72 kDa. The expected band size for MINDY4 is at 84 kDa.
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