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ProductsBack/FAM188B Antibody (aa186-235)

FAM188B Antibody (aa186-235)

LS-C205490
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMINDY4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FAM188B Antibody (aa186-235)
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84182
  • Target name
    MINDY4
  • Target description
    MINDY lysine 48 deubiquitinase 4
  • Target synonyms
    AQP-1, AQP1, C7orf67, CHIP28, FAM188B, probable ubiquitin carboxyl-terminal hydrolase MINDY-4, Aquaporin-1, Aquaporin-CHIP, MINDY deubiquitinase 4, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule, family with sequence similarity 188 member B, probable deubiquitinating enzyme MINDY-4, probable ubiquitin carboxyl-terminal hydrolase FAM188B, protein FAM188B
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MINDY4 using anti-MINDY4 antibody (A32449-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MINDY4 antigen affinity purified polyclonal antibody (Catalog # A32449-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MINDY4 at approximately 72 kDa. The expected band size for MINDY4 is at 84 kDa.
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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
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