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Immunohistochemical staining of human testis shows positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows positivity in cells in seminiferous ducts.

Anti-MKL1 Antibody

HPA030782
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMRTFA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MKL1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57591
  • Target name
    MRTFA
  • Target description
    myocardin related transcription factor A
  • Target synonyms
    BSAC, MAL, MKL, MKL1, MRTF-A, myocardin-related transcription factor A, MKL/myocardin-like protein 1, alternative protein MKL1, basic, SAP and coiled-coil domain, megakaryoblastic leukemia (translocation) 1, megakaryoblastic leukemia 1 protein, megakaryocytic acute leukemia protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ969V6
  • Protein Name
    MKL/myocardin-like protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Mkl1/MRTFA using anti-Mkl1/MRTFA antibody (A32468-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mkl1/MRTFA antigen affinity purified polyclonal antibody (Catalog # A32468-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mkl1/MRTFA at approximately 145 kDa. The expected band size for Mkl1/MRTFA is at 145 kDa.
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