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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-MMACHC Antibody

HPA027402
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMMACHC
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MMACHC Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25974
  • Target name
    MMACHC
  • Target description
    metabolism of cobalamin associated C
  • Target synonyms
    cblC, cyanocobalamin reductase / alkylcobalamin dealkylase, alkylcobalamin:glutathione S-alkyltransferase, cyanocobalamin reductase (cyanide-eliminating), methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria, methylmalonic aciduria and homocystinuria type C protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4U1
  • Protein Name
    Cyanocobalamin reductase / alkylcobalamin dealkylase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MMACHC using anti-MMACHC antibody (A03398-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMACHC antigen affinity purified polyclonal antibody (Catalog # A03398-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMACHC at approximately 32 kDa. The expected band size for MMACHC is at 32 kDa.
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