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Immunohistochemical staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.

Anti-MMP16 Antibody

HPA023693
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMMP16
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MMP16
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4325
  • Target name
    MMP16
  • Target description
    matrix metallopeptidase 16
  • Target synonyms
    C8orf57, MMP-X2, MT-MMP2, MT-MMP3, MT3-MMP, matrix metalloproteinase-16, MMP-16, MT-MMP 3, MT3MMP, MTMMP3, Putative transmembrane protein C8orf57, matrix metallopeptidase 16 (membrane-inserted), membrane-type matrix metalloproteinase 3, membrane-type-3 matrix metalloproteinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51512
  • Protein Name
    Matrix metalloproteinase-16
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MMP16 using anti-MMP16 antibody (A05065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse intestine tissue lysates, Lane 2: human SMMC-7721 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP16 antigen affinity purified polyclonal antibody (Catalog # A05065) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP16 at approximately 53KD. The expected band size for MMP16 is at 53KD.
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