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Western Blot analysis of HepG2 cells using MMP-16 Polyclonal Antibody
Western Blot analysis of HepG2 cells using MMP-16 Polyclonal Antibody
Western Blot analysis of HepG2 cells using MMP-16 Polyclonal Antibody

MMP16 Antibody

CSB-PA003330
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMMP16
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Overview

  • Supplier
    Cusabio
  • Product Name
    MMP16 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4325
  • Target name
    MMP16
  • Target description
    matrix metallopeptidase 16
  • Target synonyms
    C8orf57; matrix metallopeptidase 16 (membrane-inserted); matrix metalloproteinase-16; membrane-type matrix metalloproteinase 3; membrane-type-3 matrix metalloproteinase; MMP-16; MMP-X2; MT3MMP; MT3-MMP; MT-MMP 3; MT-MMP2; MTMMP3; MT-MMP3; Putative transmembrane protein C8orf57
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51512
  • Protein Name
    Matrix metalloproteinase-16
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MMP16 using anti-MMP16 antibody (A05065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse intestine tissue lysates, Lane 2: human SMMC-7721 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP16 antigen affinity purified polyclonal antibody (Catalog # A05065) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP16 at approximately 53KD. The expected band size for MMP16 is at 53KD.
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