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Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of red pulp.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of red pulp.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of red pulp.

Anti-MMP8 Antibody

HPA021221
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMMP8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MMP8
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4317
  • Target name
    MMP8
  • Target description
    matrix metallopeptidase 8
  • Target synonyms
    CLG1, HNC, MMP-8, PMNL-CL, neutrophil collagenase, PMN leukocyte collagenase, PMNL collagenase, collagenase 2, matrix metalloproteinase 8 (neutrophil collagenase), matrix metalloproteinase-8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22894
  • Protein Name
    Neutrophil collagenase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Lane 1: Mouse Bone marrow Lysates; Lane 2: Mouse Spleen Lysates; Lane 3: Mouse Uterus Lysates; Lane 4: Mouse Testis Lysates; Lane 5: Mouse NIH/3T3 cell Lysates; Lane 6: Rat Bone marrow Lysates; Lane 7: Rat Spleen Lysates; Lane 8: Rat Uterus Lysates; Lane 9: Rat Testis Lysates; Lane 10: Human Jurkat cell Lysates; Lane 11: Human A549 cell Lysates; Lane 12: Human HL-60 cell Lysates; Lane 13: Human MCF-7 cell Lysates; Lane 14: Human HepG2 cell Lysates. Probed with MMP8 polyclonal Antibody, unconjugated (bs-1913R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of MMP8 using anti-MMP8 antibody (PB9726). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. Lane 1: K562 Whole Cell Lysate, Lane 2: JURKAT Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP8 antigen affinity purified polyclonal antibody (Catalog # PB9726) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP8 at approximately 60KD. The expected band size for MMP8 is at 53KD.
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