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Western Blot analysis of 3T3 cells using MMP-8 Polyclonal Antibody
Western Blot analysis of 3T3 cells using MMP-8 Polyclonal Antibody
Western Blot analysis of 3T3 cells using MMP-8 Polyclonal Antibody

MMP8 Antibody

CSB-PA010208
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMMP8
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Overview

  • Supplier
    Cusabio
  • Product Name
    MMP8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4317
  • Target name
    MMP8
  • Target description
    matrix metallopeptidase 8
  • Target synonyms
    CLG1, HNC, MMP-8, PMNL-CL, neutrophil collagenase, PMN leukocyte collagenase, PMNL collagenase, collagenase 2, matrix metalloproteinase 8 (neutrophil collagenase), matrix metalloproteinase-8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22894
  • Protein Name
    Neutrophil collagenase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: Mouse Bone marrow Lysates; Lane 2: Mouse Spleen Lysates; Lane 3: Mouse Uterus Lysates; Lane 4: Mouse Testis Lysates; Lane 5: Mouse NIH/3T3 cell Lysates; Lane 6: Rat Bone marrow Lysates; Lane 7: Rat Spleen Lysates; Lane 8: Rat Uterus Lysates; Lane 9: Rat Testis Lysates; Lane 10: Human Jurkat cell Lysates; Lane 11: Human A549 cell Lysates; Lane 12: Human HL-60 cell Lysates; Lane 13: Human MCF-7 cell Lysates; Lane 14: Human HepG2 cell Lysates. Probed with MMP8 polyclonal Antibody, unconjugated (bs-1913R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of MMP8 using anti-MMP8 antibody (PB9726). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. Lane 1: K562 Whole Cell Lysate, Lane 2: JURKAT Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP8 antigen affinity purified polyclonal antibody (Catalog # PB9726) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP8 at approximately 60KD. The expected band size for MMP8 is at 53KD.
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Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of red pulp.
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