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Immunohistochemical staining of human tonsil shows strong immunoreactivity in a subset of lymphoid cells, primarily outside germinal center.
Immunohistochemical staining of human tonsil shows strong immunoreactivity in a subset of lymphoid cells, primarily outside germinal center.
Immunohistochemical staining of human tonsil shows strong immunoreactivity in a subset of lymphoid cells, primarily outside germinal center.

Anti-MMP9 Antibody

AMAB90804
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMMP9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MMP9
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL0538
  • Conjugate
    Unconjugated
  • Gene ID4318
  • Target name
    MMP9
  • Target description
    matrix metallopeptidase 9
  • Target synonyms
    CLG4B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase-9; MMP-9; type V collagenase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP14780
  • Protein Name
    Matrix metalloproteinase-9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MMP9 using anti-MMP9 antibody (PB9668). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP9 antigen affinity purified polyclonal antibody (Catalog # PB9668) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP9 at approximately 79 kDa. The expected band size for MMP9 is at 79 kDa.
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