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Anti-gelatinase B [REGA-3G12]

AB04197-1.1
Absolute Antibody
ApplicationsImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking
Product group Antibodies
TargetMMP9
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-gelatinase B [REGA-3G12]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The specificity of this antibodys original format (mouse IgG1, kappa) was evaluated by ELISA; it specifically bound to gelatinase B with a binding affinity of 2.1 x 10-9 M. This antibody detected both native and H2NPhAcHg-treated gelatinase B in an immunoblot, both in non-reducing and reducing conditions. Furthermore, this antibody was found to inhibit gelatinase B activity in vitro (Paemen et al., 1995; PMID: 8575432). The binding of the scFv version of this antibody to gelatinase B was confirmed in an ELISA. The original antibody and its F(ab)2 and scFv derivatives inhibited gelatinase B in vitro (Zhou et al., 1997; PMID: 9323036). The following scFv derivatives of this antibody were evaluated for their inhibitory effect toward gelatinase B: REGA-3G12 scFv with (His6) monomer (10 microM), REGA-3G12 scFv with (His6) dimer (5 microM), and REGA-3G12 scFv without a tag (1.25, 2.5, and 5 microM). They achieved 6.5%, 65.9%, 8.8%, 32.4%, and 44.4% inhibitory activity, respectively. Moreover, the scFv His-tag dimer variant was found to cross-react with gelatinase A with a 75% inhibitory activity (same antibody concentration) (Hu et al., 2004; PMID: 15104254). The original format of the antibody was used to detect and characterize the binding of this antibody to gelatinase B via immunoprecipitation (IP) and Western blot (WB), and the catalytic domain in the aminoterminal segment of gelatinase B was identified as its target (Martens et al., 2007; PMID: 17137715).
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    REGA-3G12
  • Gene ID4318
  • Target name
    MMP9
  • Target description
    matrix metallopeptidase 9
  • Target synonyms
    CLG4B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase-9; MMP-9; type V collagenase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP14780
  • Protein Name
    Matrix metalloproteinase-9
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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