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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-MOCS2 Antibody

HPA037680
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMOCS2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MOCS2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4338
  • Target name
    MOCS2
  • Target description
    molybdenum cofactor synthesis 2
  • Target synonyms
    MCBPE, MOCO1, MOCODB, MPTS, molybdopterin synthase catalytic subunit, molybdopterin synthase sulfur carrier subunit, molybdenum cofactor biosynthesis protein E
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO96007
  • Protein Name
    Molybdopterin synthase catalytic subunit
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MOCS2 using anti-MOCS2 antibody (A07401). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human LNCAP whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOCS2 antigen affinity purified polyclonal antibody (Catalog # A07401) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOCS2 at approximately 21 kDa. The expected band size for MOCS2 is at 21 kDa.
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Western blot All lanes: MOCS2 antibody at 1.68 ug/ml Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa
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