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Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Anti-MOGAT2 Antibody

HPA028834
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMOGAT2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MOGAT2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80168
  • Target name
    MOGAT2
  • Target description
    monoacylglycerol O-acyltransferase 2
  • Target synonyms
    DGAT2L5, DGAT2L5., MGAT2, hDC5, 2-acylglycerol O-acyltransferase 2, acyl-CoA:monoacylglycerol acyltransferase 2, diacylglycerol O-acyltransferase candidate 5, diacylglycerol acyltransferase 2-like protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ3SYC2
  • Protein Name
    2-acylglycerol O-acyltransferase 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MOGAT2 using anti-MOGAT2 antibody (A10993-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOGAT2 antigen affinity purified polyclonal antibody (Catalog # A10993-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOGAT2 at approximately 43 kDa. The expected band size for MOGAT2 is at 38 kDa.
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