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Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA663570LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA663570LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA663570LA01HU at dilution of 1:100

MOGAT2 Antibody

CSB-PA663570LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMOGAT2
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Overview

  • Supplier
    Cusabio
  • Product Name
    MOGAT2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80168
  • Target name
    MOGAT2
  • Target description
    monoacylglycerol O-acyltransferase 2
  • Target synonyms
    DGAT2L5, DGAT2L5., MGAT2, hDC5, 2-acylglycerol O-acyltransferase 2, acyl-CoA:monoacylglycerol acyltransferase 2, diacylglycerol O-acyltransferase candidate 5, diacylglycerol acyltransferase 2-like protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ3SYC2
  • Protein Name
    2-acylglycerol O-acyltransferase 2
  • Scientific Description
    Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MOGAT2 using anti-MOGAT2 antibody (A10993-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOGAT2 antigen affinity purified polyclonal antibody (Catalog # A10993-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOGAT2 at approximately 43 kDa. The expected band size for MOGAT2 is at 38 kDa.
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