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Immunohistochemical staining of human esophagus shows nuclear and cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows nuclear and cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows nuclear and cytoplasmic positivity in squamous epithelial cells.

Anti-MORC3 Antibody

HPA018406
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMORC3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MORC3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23515
  • Target name
    MORC3
  • Target description
    MORC family CW-type zinc finger 3
  • Target synonyms
    MORC family CW-type zinc finger protein 3; nuclear matrix protein 2; nuclear matrix protein NXP2; NXP2; ZCW5; ZCWCC3; zinc finger CW-type coiled-coil domain protein 3; zinc finger, CW type with coiled-coil domain 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14149
  • Protein Name
    MORC family CW-type zinc finger protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MORC3 using anti-MORC3 antibody (A06075-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MORC3 antigen affinity purified polyclonal antibody (Catalog # A06075-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MORC3 at approximately 120KD. The expected band size for MORC3 is at 107KD.
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