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WB analysis of Jurkat cells using GTX46520 MORC3 antibody at 0.2-1microg/ml.
WB analysis of Jurkat cells using GTX46520 MORC3 antibody at 0.2-1microg/ml.
WB analysis of Jurkat cells using GTX46520 MORC3 antibody at 0.2-1microg/ml.

MORC3 antibody, N-term

GTX46520
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetMORC3
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Overview

  • Supplier
    GeneTex
  • Product Name
    MORC3 antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23515
  • Target name
    MORC3
  • Target description
    MORC family CW-type zinc finger 3
  • Target synonyms
    MORC family CW-type zinc finger protein 3; nuclear matrix protein 2; nuclear matrix protein NXP2; NXP2; ZCW5; ZCWCC3; zinc finger CW-type coiled-coil domain protein 3; zinc finger, CW type with coiled-coil domain 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14149
  • Protein Name
    MORC family CW-type zinc finger protein 3
  • Scientific Description
    This gene encodes a protein that localizes to the nuclear matrix and forms nuclear bodies via an ATP-dependent mechanism. The protein is predicted to have coiled-coil and zinc finger domains and has RNA binding activity. Alternative splicing produces multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MORC3 using anti-MORC3 antibody (A06075-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MORC3 antigen affinity purified polyclonal antibody (Catalog # A06075-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MORC3 at approximately 120KD. The expected band size for MORC3 is at 107KD.
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