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Anti-mouse IL-1beta mAb (MTB2433), biotin

Anti-mouse IL-1beta mAb (MTB2433), biotin

3317-6
Mabtech
ApplicationsELISA, ELISpot Assay
Product group Antibodies
ReactivityMouse
TargetIl1b
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-mouse IL-1beta mAb (MTB2433), biotin
  • Delivery Days Customer
    9
  • Applications
    ELISA, ELISpot Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MTB2433
  • Conjugate
    Biotin
  • Gene ID16176
  • Target name
    Il1b
  • Target description
    interleukin 1 beta
  • Target synonyms
    IL-1beta, Il-1b, interleukin-1 beta, IL-1 beta, interleukin 1b
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of mouse IL-1beta in immunoassays such as ELISpot and ELISA.
  • Reactivity
    Mouse
  • UNSPSC
    12352203

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Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [HL3084] (GTX640530) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of IL1 beta using anti-IL1 beta antibody (A00101-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7(-LPS) whole cell lysates, Lane 2: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1 beta antigen affinity purified polyclonal antibody (Catalog # A00101-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1 beta at approximately 36 kDa. The expected band size for IL1 beta is at 31 kDa.
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