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Anti-mouse TNF-alpha mAbs (MT1C8/23C9), unconjugated

Anti-mouse TNF-alpha mAbs (MT1C8/23C9), unconjugated

3511-3
Mabtech
ApplicationsELISA, ELISpot Assay
Product group Antibodies
TargetTnf
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-mouse TNF-alpha mAbs (MT1C8/23C9), unconjugated
  • Delivery Days Customer
    9
  • Applications
    ELISA, ELISpot Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MT1C8/23C9
  • Conjugate
    Unconjugated
  • Gene ID21926
  • Target name
    Tnf
  • Target description
    tumor necrosis factor
  • Target synonyms
    DIF, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tnfa, Tnfsf1a, Tnlg1f, tumor necrosis factor, cachectin, tumor necrosis factor ligand 1f, tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
  • Host
    Rat
  • Isotype
    IgG2a
  • Scientific Description
    Validated for our assays. - These monoclonal antibodies enable specific detection of mouse TNF-alpha in immunoassays such as ELISpot, FluoroSpot, and ELISA.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 25KD. The expected band size for TNF alpha is at 25KD.
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Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated (bs-2081R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
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