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Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated (bs-2081R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated (bs-2081R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated (bs-2081R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

TNF alpha Polyclonal Antibody

BS-2081R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTnf
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    TNF alpha Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID21926
  • Target name
    Tnf
  • Target description
    tumor necrosis factor
  • Target synonyms
    cachectin; DI; DIF; Tn; TNF-; Tnfa; TNF-a; TNFalpha; TNF-alpha; Tnfs; Tnfsf1a; TNFSF2; Tnlg1f; tumor necrosis factor; tumor necrosis factor ligand 1f; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06804
  • Protein Name
    Tumor necrosis factor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Biliary antibiotics irrigation for E. coli-induced chronic proliferative cholangitis and hepatolithiasis: A pathophysiological study in rabbits. Ma WJ et al., 2020 Jun, Clin Res Hepatol Gastroenterol
    Read more
  • Long-term Western diet intake leads to dysregulated bile acid signaling and dermatitis with Th2 and Th17 pathway features in mice. Jena PK et al., 2019 Jul, J Dermatol Sci
    Read more
  • Anti-inflammatory effects of quercetin in a mouse model of MC903-induced atopic dermatitis. Hou DD et al., 2019 Sep, Int Immunopharmacol
    Read more
  • MCC950 attenuated early brain injury by suppressing NLRP3 inflammasome after experimental SAH in rats. Luo Y et al., 2019 Mar, Brain Res Bull
    Read more
  • Bradykinin contributes to immune liver injury via B2R receptor-mediated pathways in trichloroethylene sensitized mice: A role in Kupffer cell activation. Zhang J et al., 2019 Mar 1, Toxicology
    Read more
  • Selective IKK2 inhibitor IMD0354 disrupts NF-kappaB signaling to suppress corneal inflammation and angiogenesis. Lennikov A et al., 2018 May, Angiogenesis
    Read more
  • Local administration of nuclear factor of activated T cells (NFAT) c1 inhibitor to suppress early resorption and inflammation induced by bone morphogenetic protein-2. Kim RY et al., 2018 May, J Biomed Mater Res A
    Read more
  • Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation. Wang X et al., 2017 Nov, Cytokine
    Read more
  • Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation. Li X et al., 2017 Oct, Mol Med Rep
    Read more
  • LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4. Han S et al., 2017 Jun 22, J Exp Clin Cancer Res
    Read more

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Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 25KD. The expected band size for TNF alpha is at 25KD.
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