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Anti-Mouse/Rat CUX1 Antibody

144-02213
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetCUX1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Mouse/Rat CUX1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1523
  • Target name
    CUX1
  • Target description
    cut like homeobox 1
  • Target synonyms
    CASP; CCAAT displacement protein; CDP; CDP/Cut; CDP/Cux p200; CDP1; Clox; COY1; cut homolog; CUTL1; CUX; Cux/CDP; CUX1 gene Alternatively Spliced Product; GDDI; golgi integral membrane protein 6; GOLIM6; Homeobox protein cut-like 1; homeobox protein cux-1; mutant CUX1; Nbla10317; p100; p110; p200; p75; protein CASP; putative protein product of Nbla10317
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP39880
  • Protein Name
    Homeobox protein cut-like 1
  • Scientific Description
    CUX1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CUX1 using anti-CUX1 antibody (A01653-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUX1 antigen affinity purified polyclonal antibody (Catalog # A01653-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CUX1 at approximately 200 kDa. The expected band size for CUX1 is at 164 kDa.
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