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Western Blot analysis of 293T cells using CUX1 Polyclonal Antibody
Western Blot analysis of 293T cells using CUX1 Polyclonal Antibody
Western Blot analysis of 293T cells using CUX1 Polyclonal Antibody

CUX1/CUX1 Antibody

CSB-PA001549
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCUX1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CUX1/CUX1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1523
  • Target name
    CUX1
  • Target description
    cut like homeobox 1
  • Target synonyms
    CASP, CDP, CDP/Cut, CDP1, COY1, CUTL1, CUX, Clox, Cux/CDP, GDDI, GOLIM6, NEDDMS, Nbla10317, p100, p110, p200, p75, protein CASP, Homeobox protein cut-like 1, CCAAT displacement protein, CDP/Cux p200, CUX1 gene Alternatively Spliced Product, cut homolog, golgi integral membrane protein 6, homeobox protein cux-1, mutant CUX1, putative protein product of Nbla10317
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP39880
  • Protein Name
    Homeobox protein cut-like 1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CUX1 using anti-CUX1 antibody (A01653-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUX1 antigen affinity purified polyclonal antibody (Catalog # A01653-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CUX1 at approximately 200 kDa. The expected band size for CUX1 is at 164 kDa.
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