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Immunohistochemical staining of human cerebral cortex, kidney, skin and testis using Anti-MPHOSPH10 antibody HPA035059 (A) shows similar protein distribution across tissues to independent antibody HPA035060 (B).
Immunohistochemical staining of human cerebral cortex, kidney, skin and testis using Anti-MPHOSPH10 antibody HPA035059 (A) shows similar protein distribution across tissues to independent antibody HPA035060 (B).
Immunohistochemical staining of human cerebral cortex, kidney, skin and testis using Anti-MPHOSPH10 antibody HPA035059 (A) shows similar protein distribution across tissues to independent antibody HPA035060 (B).

Anti-MPHOSPH10 Antibody

HPA035059
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMPHOSPH10
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MPHOSPH10 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10199
  • Target name
    MPHOSPH10
  • Target description
    M-phase phosphoprotein 10
  • Target synonyms
    CT90, MPP10, MPP10P, PPP1R106, U3 small nucleolar ribonucleoprotein MPP10, U3 small nucleolar ribonucleoprotein, m phase phosphoprotein 10, protein phosphatase 1, regulatory subunit 106
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00566
  • Protein Name
    U3 small nucleolar ribonucleoprotein protein MPP10
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MPHOSPH10 using anti-MPHOSPH10 antibody (A11510-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPHOSPH10 antigen affinity purified polyclonal antibody (Catalog # A11510-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPHOSPH10 at approximately 120 kDa. The expected band size for MPHOSPH10 is at 120 kDa.
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