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Western Blot analysis of Jurkat cells using MPP10 Polyclonal Antibody
Western Blot analysis of Jurkat cells using MPP10 Polyclonal Antibody
Western Blot analysis of Jurkat cells using MPP10 Polyclonal Antibody

MPHOSPH10 Antibody

CSB-PA004713
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMPHOSPH10
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Overview

  • Supplier
    Cusabio
  • Product Name
    MPHOSPH10 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10199
  • Target name
    MPHOSPH10
  • Target description
    M-phase phosphoprotein 10
  • Target synonyms
    CT90, MPP10, MPP10P, PPP1R106, U3 small nucleolar ribonucleoprotein MPP10, U3 small nucleolar ribonucleoprotein, m phase phosphoprotein 10, protein phosphatase 1, regulatory subunit 106
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00566
  • Protein Name
    U3 small nucleolar ribonucleoprotein protein MPP10
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MPHOSPH10 using anti-MPHOSPH10 antibody (A11510-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPHOSPH10 antigen affinity purified polyclonal antibody (Catalog # A11510-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPHOSPH10 at approximately 120 kDa. The expected band size for MPHOSPH10 is at 120 kDa.
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TargetMPHOSPH10
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Immunohistochemical staining of human cerebral cortex, kidney, skin and testis using Anti-MPHOSPH10 antibody HPA035059 (A) shows similar protein distribution across tissues to independent antibody HPA035060 (B).
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