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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.

Anti-MPZL1 Antibody

HPA026966
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMPZL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MPZL1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9019
  • Target name
    MPZL1
  • Target description
    myelin protein zero like 1
  • Target synonyms
    MPZL1b, PZR, PZR1b, PZRa, PZRb, myelin protein zero-like protein 1, immunoglobulin family transmembrane protein, protein zero related
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95297
  • Protein Name
    Myelin protein zero-like protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MPZL1 using anti-MPZL1 antibody (A06667-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPZL1 antigen affinity purified polyclonal antibody (Catalog # A06667-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPZL1 at approximately 29-37 kDa. The expected band size for MPZL1 is at 29 kDa.
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