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Mouse kidney lysates probed with MPZL Polyclonal Antibody, Unconjugated (bs-17744R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse kidney lysates probed with MPZL Polyclonal Antibody, Unconjugated (bs-17744R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse kidney lysates probed with MPZL Polyclonal Antibody, Unconjugated (bs-17744R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

MPZL Polyclonal Antibody

BS-17744R
Bioss
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat, Sheep
TargetMPZL1
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Overview

  • Supplier
    Bioss
  • Product Name
    MPZL Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9019
  • Target name
    MPZL1
  • Target description
    myelin protein zero like 1
  • Target synonyms
    MPZL1b, PZR, PZR1b, PZRa, PZRb, myelin protein zero-like protein 1, immunoglobulin family transmembrane protein, protein zero related
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95297
  • Protein Name
    Myelin protein zero-like protein 1
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MPZL1 using anti-MPZL1 antibody (A06667-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPZL1 antigen affinity purified polyclonal antibody (Catalog # A06667-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPZL1 at approximately 29-37 kDa. The expected band size for MPZL1 is at 29 kDa.
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