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Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.
Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.
Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.

Anti-MR1 Antibody

HPA048304
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MR1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3140
  • Target name
    MR1
  • Target description
    major histocompatibility complex, class I-related
  • Target synonyms
    HLALS, major histocompatibility complex class I-related protein 1, MHC class I-like antigen MR-1, MHC class I-related protein, MHC class I-related protein 1, MHC class-I related-gene protein, major histocompatibility complex class I-related gene protein, major histocompatibility complex, class I-like sequence
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ95460
  • Protein Name
    Major histocompatibility complex class I-related protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MR1 using anti-MR1 antibody (A00618-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MR1 antigen affinity purified polyclonal antibody (Catalog # A00618-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MR1 at approximately 40KD. The expected band size for MR1 is at 43KD.
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