Figure 3. IHC analysis of S100A8 using anti-S100A8 antibody (PB9742). S100A8 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-S100A8 Antibody (PB9742) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of S100A8 using anti-S100A8 antibody (PB9742). S100A8 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-S100A8 Antibody (PB9742) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-MRP8/S100A8 Antibody Picoband(r)
PB9742-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetS100a8
Overview
- SupplierBoster Bio
- Product NameAnti-MRP8/S100A8 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID20201
- Target nameS100a8
- Target descriptionS100 calcium binding protein A8 (calgranulin A)
- Target synonyms60B8Ag, B8Ag, CFAg, CP-10, Caga, MRP8, p8, protein S100-A8, MRP-8, S100 calcium-binding protein A8, calgranulin-A, chemotactic S100 protein, chemotactic cytokine CP-10, leukocyte L1 complex light chain, migration inhibitory factor-related protein 8, pro-inflammatory S100 cytokine
- HostRabbit
- IsotypeIgG
- Protein IDP27005
- Protein NameProtein S100-A8
- Scientific DescriptionBoster Bio Anti-MRP8/S100A8 Antibody Picoband® catalog # PB9742. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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