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Anti-MRPS18B Antibody

144-12085
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMRPS18B
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-MRPS18B Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID28973
  • Target name
    MRPS18B
  • Target description
    mitochondrial ribosomal protein S18B
  • Target synonyms
    C6orf14, HSPC183, HumanS18a, MRP-S18-2, MRPS18-2, PTD017, S18amt, mS40, small ribosomal subunit protein mS40, 28S ribosomal protein S18-2, mitochondrial, 28S ribosomal protein S18b, mitochondrial, MRP-S18-b, S18mt-b, mitochondrial ribosomal protein S18-2, mitochondrial small ribosomal subunit protein bS18b, mitochondrial small ribosomal subunit protein bS18m-B, mitochondrial small ribosomal subunit protein mS40, mrps18-b, small ribosomal subunit protein bS18b
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y676
  • Protein Name
    Small ribosomal subunit protein mS40
  • Scientific Description
    MRPS18B Polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MRPS18B using anti-MRPS18B antibody (A12773-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human SH-SY5Y whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRPS18B antigen affinity purified polyclonal antibody (Catalog # A12773-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRPS18B at approximately 26 kDa. The expected band size for MRPS18B is at 29 kDa.
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