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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells, cells in molecular layer and granular layer.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells, cells in molecular layer and granular layer.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells, cells in molecular layer and granular layer.

Anti-MRPS18B Antibody

HPA043485
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMRPS18B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MRPS18B Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID28973
  • Target name
    MRPS18B
  • Target description
    mitochondrial ribosomal protein S18B
  • Target synonyms
    C6orf14, HSPC183, HumanS18a, MRP-S18-2, MRPS18-2, PTD017, S18amt, mS40, small ribosomal subunit protein mS40, 28S ribosomal protein S18-2, mitochondrial, 28S ribosomal protein S18b, mitochondrial, MRP-S18-b, S18mt-b, mitochondrial ribosomal protein S18-2, mitochondrial small ribosomal subunit protein bS18b, mitochondrial small ribosomal subunit protein bS18m-B, mitochondrial small ribosomal subunit protein mS40, mrps18-b, small ribosomal subunit protein bS18b
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y676
  • Protein Name
    Small ribosomal subunit protein mS40
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Immunohistochemical staining of human cerebellum shows weak cytoplasmic positivity in Purkinje cells, cells in molecular layer and granular layer).
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Figure 1. Western blot analysis of MRPS18B using anti-MRPS18B antibody (A12773-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human SH-SY5Y whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRPS18B antigen affinity purified polyclonal antibody (Catalog # A12773-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRPS18B at approximately 26 kDa. The expected band size for MRPS18B is at 29 kDa.
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