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Anti-MSH6 [RAB-C105]

AB01811-1.1-BT
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation
Product group Antibodies
TargetMSH6
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-MSH6 [RAB-C105]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: FC; spiked-IP; IP-MS; IF.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-C105
  • Gene ID2956
  • Target name
    MSH6
  • Target description
    mutS homolog 6
  • Target synonyms
    DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; HNPCC5; HSAP; MMRCS3; mutS protein homolog 6; mutS-alpha 160 kDa subunit; p160; sperm-associated protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP52701
  • Protein Name
    DNA mismatch repair protein Msh6
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MSH6 using anti-MSH6 antibody (A00553-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SKOV3 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH6 antigen affinity purified polyclonal antibody (Catalog # A00553-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSH6 at approximately 160KD. The expected band size for MSH6 is at 160KD.
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