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MSH6 Antibody (clone ABT-MSH6)

LS-C744537
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMSH6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MSH6 Antibody (clone ABT-MSH6)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Use Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH 8.0 Recommended IHC controls tissues are Tonsil, Appendix, Colon, Colon carcinoma with intact PMS2 expression. Resulting localization is Nuclear
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:200 - 1:400), WB (1:200 - 1:400) Use Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH 8.0 Recommended IHC controls tissues are Tonsil, Appendix, Colon, Colon carcinoma with intact PMS2 expression. Resulting localization is Nuclear
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ABT-MSH6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2956
  • Target name
    MSH6
  • Target description
    mutS homolog 6
  • Target synonyms
    DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; HNPCC5; HSAP; MMRCS3; mutS protein homolog 6; mutS-alpha 160 kDa subunit; p160; sperm-associated protein
  • Host
    Mouse
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MSH6 using anti-MSH6 antibody (A00553-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SKOV3 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH6 antigen affinity purified polyclonal antibody (Catalog # A00553-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSH6 at approximately 160KD. The expected band size for MSH6 is at 160KD.
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