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Anti-MSN Antibody

130-10694
RayBiotech
ApplicationsELISA
Product group Antibodies
TargetMSN
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-MSN Antibody
  • Delivery Days Customer
    16
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4G11-G6-E8
  • Gene ID4478
  • Target name
    MSN
  • Target description
    moesin
  • Target synonyms
    epididymis luminal protein 70; HEL70; IMD50; membrane-organizing extension spike protein; moesin
  • Host
    Mouse
  • Protein IDP26038
  • Protein Name
    Moesin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MSN using anti-MSN antibody (A00766-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSN antigen affinity purified polyclonal antibody (Catalog # A00766-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSN at approximately 78KD. The expected band size for MSN is at 66KD.
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