Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Moesin antibody [C2C3], C-term (GTX101708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Moesin antibody [C2C3], C-term detects MSN protein by Western blot analysis. A. 30 μg GL261 whole cell lysate/extract B. 30 μg C8D30 whole cell lysate/extract C. 30 μg NIH-3T3 whole cell lysate/extract D. 30 μg BCL-1 whole cell lysate/extract E. 30 μg Raw264.7 whole cell lysate/extract F. 30 μg C2C12 whole cell lysate/extract 7.5 % SDS-PAGE Moesin antibody [C2C3], C-term (GTX101708) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX101708/GTX101708_40198_WB_M_w_23060100_741.webp "Moesin antibody [C2C3], C-term detects MSN protein by Western blot analysis. A. 30 μg GL261 whole cell lysate/extract B. 30 μg C8D30 whole cell lysate/extract C. 30 μg NIH-3T3 whole cell lysate/extract D. 30 μg BCL-1 whole cell lysate/extract E. 30 μg Raw264.7 whole cell lysate/extract F. 30 μg C2C12 whole cell lysate/extract 7.5 % SDS-PAGE Moesin antibody [C2C3], C-term (GTX101708) dilution: 1:1000")
![Immunoprecipitation of Moesin protein from A431 whole cell extracts using 5 μg of Moesin antibody [C2C3], C-term (GTX101708). Western blot analysis was performed using Moesin antibody [C2C3], C-term (GTX101708). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101708/GTX101708_40198_20150413_IP_w_23060100_794.webp "Immunoprecipitation of Moesin protein from A431 whole cell extracts using 5 μg of Moesin antibody [C2C3], C-term (GTX101708). Western blot analysis was performed using Moesin antibody [C2C3], C-term (GTX101708). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![Moesin antibody [C2C3], C-term detects Moesin protein at cell membrane in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Moesin antibody [C2C3], C-term (GTX101708) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101708/GTX101708_40198_20160301_IHC-P_R_w_23060100_326.webp "Moesin antibody [C2C3], C-term detects Moesin protein at cell membrane in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Moesin antibody [C2C3], C-term (GTX101708) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Moesin antibody [C2C3], C-term (GTX101708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Moesin antibody [C2C3], C-term
GTX101708
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMSN
Overview
- SupplierGeneTex
- Product NameMoesin antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.21 mg/ml
- ConjugateUnconjugated
- Gene ID4478
- Target nameMSN
- Target descriptionmoesin
- Target synonymsHEL70, IMD50, moesin, epididymis luminal protein 70, membrane-organizing extension spike protein
- HostRabbit
- IsotypeIgG
- Protein IDP26038
- Protein NameMoesin
- Scientific DescriptionMoesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Alshafie W, Ayoubi R, Fotouhi M, et al. Identification of high-performing antibodies for Moesin for use in Western Blot, immunoprecipitation, and immunofluorescence. F1000Res. 2023,12:172. doi: 10.12688/f1000research.130126.3Read this paper
- Chang YT, Peng HY, Hu CM, et al. Pancreatic cancer-derived small extracellular vesical Ezrin regulates macrophage polarization and promotes metastasis. Am J Cancer Res. 2020,10(1):12-37.Read this paper
- Ogiwara K, Takahashi T. A Dual Role for Melatonin in Medaka Ovulation: Ensuring Prostaglandin Synthesis and Actin Cytoskeleton Rearrangement in Follicular Cells. Biol Reprod. 2016,94(3):64. doi: 10.1095/biolreprod.115.133827Read this paper
- Babina IS, McSherry EA, Donatello S, et al. A novel mechanism of regulating breast cancer cell migration via palmitoylation-dependent alterations in the lipid raft affiliation of CD44. Breast Cancer Res. 2014,16(1):R19. doi: 10.1186/bcr3614Read this paper
- Chen YH, Chou HC, Lin ST, et al. Effect of high glucose on secreted proteome in cultured retinal pigmented epithelium cells: its possible relevance to clinical diabetic retinopathy. J Proteomics. 2012,77:111-28. doi: 10.1016/j.jprot.2012.07.014Read this paper
Datasheet
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