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Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.

Anti-MSRB3 Antibody

HPA014432
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetMSRB3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MSRB3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID253827
  • Target name
    MSRB3
  • Target description
    methionine sulfoxide reductase B3
  • Target synonyms
    DFNB74; methionine-R-sulfoxide reductase B3; methionine-R-sulfoxide reductase B3, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IXL7
  • Protein Name
    Methionine-R-sulfoxide reductase B3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MSRB3 using anti-MSRB3 antibody (A06632-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSRB3 antigen affinity purified polyclonal antibody (Catalog # A06632-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSRB3 at approximately 21 kDa. The expected band size for MSRB3 is at 21 kDa.
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