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Western blot All lanes: MSRB3 antibody at 4.61 ug/ml Lane 1: Mouse large intestine tissue Lane 2: Mouse lung tissue Lane 3: Mouse gonadal tissue Lane 4: Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa
Western blot All lanes: MSRB3 antibody at 4.61 ug/ml Lane 1: Mouse large intestine tissue Lane 2: Mouse lung tissue Lane 3: Mouse gonadal tissue Lane 4: Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa
Western blot All lanes: MSRB3 antibody at 4.61 ug/ml Lane 1: Mouse large intestine tissue Lane 2: Mouse lung tissue Lane 3: Mouse gonadal tissue Lane 4: Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa

MSRB3 Antibody

CSB-PA810290DSR1HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetMSRB3
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Overview

  • Supplier
    Cusabio
  • Product Name
    MSRB3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID253827
  • Target name
    MSRB3
  • Target description
    methionine sulfoxide reductase B3
  • Target synonyms
    DFNB74; methionine-R-sulfoxide reductase B3; methionine-R-sulfoxide reductase B3, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IXL7
  • Protein Name
    Methionine-R-sulfoxide reductase B3
  • Scientific Description
    Catalyzes the reduction of free and protein-bound methionine sulfoxide to methionine. Isoform 2 is essential for hearing.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MSRB3 using anti-MSRB3 antibody (A06632-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSRB3 antigen affinity purified polyclonal antibody (Catalog # A06632-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSRB3 at approximately 21 kDa. The expected band size for MSRB3 is at 21 kDa.
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