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Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Anti-MSX2 Antibody

HPA005652
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMSX2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MSX2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4488
  • Target name
    MSX2
  • Target description
    msh homeobox 2
  • Target synonyms
    CRS2; FPP; homeobox protein Hox-8; homeobox protein MSX-2; HOX8; MSH; msh homeo box 2; msh homeobox homolog 2; PFM; PFM1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35548
  • Protein Name
    Homeobox protein MSX-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Hox8/MSX2 using anti-Hox8/MSX2 antibody (A01783-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hox8/MSX2 antigen affinity purified polyclonal antibody (Catalog # A01783-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hox8/MSX2 at approximately 37 kDa. The expected band size for Hox8/MSX2 is at 29 kDa.
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