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ProductsBack/MSX2 / MSH Antibody

MSX2 / MSH Antibody

LS-C760962
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMSX2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MSX2 / MSH Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:100), WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4488
  • Target name
    MSX2
  • Target description
    msh homeobox 2
  • Target synonyms
    CRS2, FPP, HOX8, MSH, PFM, PFM1, homeobox protein MSX-2, homeobox protein Hox-8, msh homeo box 2, msh homeobox homolog 2
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Hox8/MSX2 using anti-Hox8/MSX2 antibody (A01783-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hox8/MSX2 antigen affinity purified polyclonal antibody (Catalog # A01783-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hox8/MSX2 at approximately 37 kDa. The expected band size for Hox8/MSX2 is at 29 kDa.
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