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Anti-MT-ND4 Antibody

144-65260
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetND4
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-MT-ND4 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Gene ID4538
  • Target name
    ND4
  • Target description
    NADH dehydrogenase subunit 4
  • Target synonyms
    MTND4; NADH dehydrogenase subunit 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP03905
  • Protein Name
    NADH-ubiquinone oxidoreductase chain 4
  • Scientific Description
    MT-ND4 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MT-ND4 using anti-MT-ND4 antibody (A04180-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates Lane 2: human HepG2 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Caco-2 whole cell lysates Lane 5: human U2OS whole cell lysates Lane 6: human A549 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT-ND4 antigen affinity purified polyclonal antibody (Catalog # A04180-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MT-ND4 at approximately 36KD. The expected band size for MT-ND4 is at 52KD.
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