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Immunohistochemical staining of human Heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human Heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human Heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.

Anti-MT-ND4 Antibody

HPA053928
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetND4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MT-ND4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4538
  • Target name
    ND4
  • Target description
    NADH dehydrogenase subunit 4
  • Target synonyms
    MTND4; NADH dehydrogenase subunit 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP03905
  • Protein Name
    NADH-ubiquinone oxidoreductase chain 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MT-ND4 using anti-MT-ND4 antibody (A04180-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates Lane 2: human HepG2 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Caco-2 whole cell lysates Lane 5: human U2OS whole cell lysates Lane 6: human A549 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT-ND4 antigen affinity purified polyclonal antibody (Catalog # A04180-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MT-ND4 at approximately 36KD. The expected band size for MT-ND4 is at 52KD.
Product group Antibodies
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Anti-MT-ND4 Antibody Picoband(r)A04180-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetND4
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