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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Anti-MTF1 Antibody

HPA028689
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Chip-exoSeq
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMTF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MTF1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Chip-exoSeq
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4520
  • Target name
    MTF1
  • Target description
    metal regulatory transcription factor 1
  • Target synonyms
    metal regulatory transcription factor 1; metal-responsive transcription factor 1; MRE-binding transcription factor; MRE-binding transcription factor-1; MTF-1; transcription factor MTF-1; zinc regulatory factor; ZRF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14872
  • Protein Name
    Metal regulatory transcription factor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MTF1 using anti-MTF1 antibody (A04733-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTF1 antigen affinity purified polyclonal antibody (Catalog # A04733-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTF1 at approximately 65 kDa. The expected band size for MTF1 is at 81 kDa.
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