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ProductsBack/MTF-1 Antibody (aa2-164)

MTF-1 Antibody (aa2-164)

LS-C374597
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMTF1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MTF-1 Antibody (aa2-164)
  • Delivery Days Customer
    23
  • Application Supplier Note
    ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Applications
    Western Blot
  • Applications Supplier
    WB ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4520
  • Target name
    MTF1
  • Target description
    metal regulatory transcription factor 1
  • Target synonyms
    MTF-1, ZRF, metal regulatory transcription factor 1, MRE-binding transcription factor, MRE-binding transcription factor-1, metal-responsive transcription factor 1, transcription factor MTF-1, zinc regulatory factor
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MTF1 using anti-MTF1 antibody (A04733-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTF1 antigen affinity purified polyclonal antibody (Catalog # A04733-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTF1 at approximately 65 kDa. The expected band size for MTF1 is at 81 kDa.
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